© Springer Nature Switzerland AG 2021
C. S. Foster et al. (eds.)Uveitishttps://doi.org/10.1007/978-3-030-52974-1_46

46. Toxoplasmosis

Artur Filipowicz1   and Miriam Baron Barshak2
(1)
Massachusetts Eye Research and Surgery Institution, Waltham, MA, USA
(2)
Department of Medicine, Massachusetts General Hospital, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA, USA
 
Keywords
UveitisToxoplasmosis

Overview

  • Definition
    • A protozoal disease caused by Toxoplasma gondii, contracted from cat feces, consumption of unfiltered water, or undercooked meat (especially pork, lamb, and venison). Infection can also be acquired via vertical transmission (from mother to fetus) and via receipt of an organ transplanted from an infected donor

    • Up to a third of the world’s population is infected, but only a very small percentage manifest symptomatically

    • Ocular toxoplasmosis is the number one cause of infectious posterior uveitis in the world (>80% in some regions)

    • While chorioretinitis is common in all hosts, systemic disease is exceedingly rare in immunocompetent hosts

  • Symptoms
    • Blurry vision

    • Floaters

    • Pain (from anterior uveitis and/or scleral involvement of retinochoroiditis)

    • Redness

    • Photophobia

    • Photopsia

  • Laterality
    • Unilateral or bilateral (but simultaneous bilateral inflammation is extremely rare)

  • Course
    • Retinochoroiditis is self-limited over 2–4 weeks in immunocompetent hosts but can recur in two-thirds of patients

    • Visual prognosis is favorable unless lesion affects macula or optic nerve; surprisingly, congenital macular scar may be associated with relatively good vision

    • Much more aggressive in immunocompromised hosts

  • Age of onset
    • All ages affected, but more severe in older patients

    • Seroconversion increases with age: 5–30% of 10–19 years old are seropositive for T. gondii, and this goes up to 70% in those older than 50

  • Gender/race
    • No gender or racial predilection

    • Higher prevalence in tropical areas close to sea level

  • Systemic association
    • Congenital toxoplasmosis
      • Incidence of primary infection during pregnancy is <1%; among these, transplacental transmission risk increases with gestational age at the time of maternal infection, up to ~70% at 36 weeks
        • More severe clinical sequelae for the fetus when contracted early on in pregnancy (may lead to spontaneous abortion)

      • No risk of congenital infection from reactivation of latent toxoplasmosis during pregnancy in immunocompetent hosts; very small to no risk in immunocompromised hosts

      • Retinochoroiditis is the most common manifestation: often bilateral with macular involvement (15–40%)

      • Cerebrospinal fluid pleocytosis and elevated proteins (20%)

      • Intracranial calcifications (10%)

      • Anemia, thrombocytopenia, and jaundice at birth

      • Mental retardation, seizures, spasticity, sensorineural hearing loss are some severe neurologic sequelae

    • Acquired toxoplasmosis
      • Immunocompetent patients: only 10–20% ever develop symptoms, which are usually mild and nonspecific, and self-limited over 2–4 weeks
        • Lymphadenopathy, with mono-like illness: headache, malaise, sore throat, fatigue, fever, and night sweats

        • More severe (but unusual) symptoms: meningismus, meningoencephalitis, myalgias, arthralgias, abdominal pain, and maculopapular rash sparing palms and soles

        • Potentially lethal (but very rare) complications: encephalopathy, pneumonitis, myocarditis, polymyositis, hepatitis, and splenomegaly

      • Immunocompromised patients: many develop rapidly fatal disease if untreated
        • CNS disease (50%): encephalopathy, meningoencephalitis, and mass lesions

        • Myocarditis

        • Pneumonitis

        • Disseminated disease with septic shock

Exam: Ocular

Anterior Segment

  • Mild to severe granulomatous or non-granulomatous uveitis

  • Elevated IOP (10–15%)

Posterior Segment

  • Classic appearance (“headlight in the fog”): fluffy, focal necrotizing retinitis or retinochoroiditis adjacent to a variably pigmented chorioretinal scar, with significant overlying vitritis
    • Lesion size ranges from 1/10 disc diameters to large enough to span two quadrants of the fundus

    • Recurrent lesions are typically single and contiguous with old inactive scars, but can also recur at sites distant from the primary lesion or in the fellow eye

    • Vascular occlusion can occur if lesion involves vessels

    • Healed lesions are characterized by peripheral hyperpigmentation and central atrophy exposing the choroid and sclera; tractional bands may form between one scar to a neighboring scar or the optic disc; may be complicated by proliferative vitreoretinopathy and choroidal neovascularization

  • Vascular sheathing typically involving veins but sometimes arteries (Kyrieleis arteriolitis)

  • Optic atrophy

  • Tractional or exudative retinal detachment

Atypical Forms

  • Anterior uveitis without evidence of retinochoroiditis

  • Punctate outer retinal toxoplasmosis

  • Neuroretinitis

  • Isolated optic neuritis

  • Multifocal retinochoroiditis simulating acute retinal necrosis (ARN) (immunocompromised or recently acquired)

  • Panophthalmitis (immunocompromised)

Exam: Systemic

  • Enlarged lymph nodes (cervical [symmetrical, nontender] > suboccipital > supraclavicular > axillary > inguinal > mediastinal)

  • Abdominal tenderness, hepatosplenomegaly

  • Maculopapular rash (sparing palms and soles)

  • Altered mental status, cerebellar signs, cranial nerve palsies, sensory abnormalities, weakness

Imaging

  • OCT
    • Active lesion: hyperreflective retinal layers with posterior optical shadowing and overlying posterior hyaloid thickening

    • Healed lesion: atrophic retina with hyperreflectivity of the underlying choroid

  • FA
    • Active lesion: early central hypofluorescence followed by late leakage from the margins; adjacent retinal vascular leakage

    • Healed lesion: variable early fluorescence depending on RPE proliferation or atrophy; late staining of margins

  • ICG
    • Active lesion: early hyperfluorescence or hypofluorescence with late hyperfluorescence

    • Healed lesion: early and late hypofluorescence

Laboratory and Radiographic Testing

  • Serum or ocular fluid anti–T. gondii IgG and IgM titers
    • In acute infection, IgM should be positive within a week of developing symptoms and persist for several weeks

    • IgG develops over the next 2–3 weeks and typically persists for life

    • Patients with positive IgM and negative IgG may have acute infection but may also have false-positive IgM results due to cross-reactivity with rheumatoid factor, antinuclear antibodies, or nonspecific binding in vitro. These patients should have repeat serologies checked 2–3 weeks later to assess for development of IgG, which confirms true acute infection

    • Patients with symptoms for more than 2 weeks who lack both toxoplasma IgG and IgM likely do not have toxoplasmosis

    • In reactivation disease, serum IgM antibodies are typically absent, while IgG antibodies are present

    • Goldmann-Witmer coefficient
      $$ \mathrm{C}=\frac{\mathrm{Antibody}\kern0.17em \mathrm{titer}\kern0.17em \mathrm{in}\kern0.17em \mathrm{aqueous}\kern0.17em \mathrm{humor}}{\mathrm{Antibody}\kern0.17em \mathrm{titer}\kern0.17em \mathrm{in}\kern0.17em \mathrm{serum}}\times \frac{\mathrm{Globulin}\kern0.17em \mathrm{titer}\kern0.17em \mathrm{in}\kern0.17em \mathrm{serum}}{\mathrm{Globulin}\kern0.17em \mathrm{titer}\kern0.17em \mathrm{in}\kern0.17em \mathrm{aqueous}\kern0.17em \mathrm{humor}} $$

      0.5–2: No anti-toxoplasma intraocular antibody (Ab) production

      2–4: Possible intraocular Ab production

      >4: Definitive intraocular Ab production

  • Serum and ocular fluid T. gondii DNA PCR
    • Done in suspicious cases with negative serologic testing

  • MRI or CT of brain

  • Histopathology: tachyzoites or cysts may be seen in lymph nodes, brain tissue, or other infected organs

Differential Diagnosis

  • Congenital disease: TORCH infections

  • Endogenous fungal endophthalmitis
    • Candidiasis

    • Aspergillosis

    • Blastomycosis

  • Subretinal abscess from endogenous bacterial endophthalmitis

  • Viral retinitis

  • Syphilis

  • Tuberculosis

  • Toxocariasis

  • Sarcoidosis

  • Lymphocytic choriomeningitis virus (LCMV)

Treatment

Treat at least 4–6 weeks, then reevaluate; consult Infectious Disease for pediatric dosing
  • “Classic” therapy (nonpregnant adults)
    • Pyrimethamine: 100 mg on day 1, then 25–50 mg daily
      • Access to pyrimethamine in the United States is currently difficult— it must be obtained through a program administered by the manufacturer or from a compounding pharmacy (www.​daraprimdirect.​com/​how-to-prescribe). If this cannot be done promptly, then trimethoprim–sulfamethoxazole should be used until pyrimethamine is obtained

    • Sulfadiazine: 2 g loading dose on day 1, then 500 mg–1 g QID

    • Folinic acid (leucovorin): 5–25 mg/d based on neutrophil count

    • Congenital toxoplasmosis in newborn is treated 6–24 months

    • Prophylaxis in HIV
      • Pyrimethamine 25–75 mg daily

      • Sulfadiazine 0.5–1 g QID

      • Folinic acid 5–25 mg daily

  • Trimethoprim/sulfamethoxazole
    • 160/800 mg BID, then every other day for 1 year

    • May be less effective than classic therapy, but a good alternative when retinochoroiditis is not threatening posterior pole or optic nerve

    • Can be used in late second or third trimester for maternal and fetal infections

  • Atovaquone, preferably with pyrimethamine but alone if unable to give pyrimethamine
    • 1500 mg BID

    • Give with food to increase bioavailability

    • Excellent choice when patient has sulfa allergy

    • May need to treat for at least 3 months if giving without pyrimethamine

  • Clindamycin
    • 300 mg q6–8 h

    • Intravitreal 1 mg/0.1 ml can be used in conjunction with systemic therapy when vision is threatened

  • Azithromycin
    • 0.5–1 g daily

  • Spiramycin
    • 1 g TID

    • Drug of choice during early pregnancy (<18 weeks gestation) due to concerns about teratogenicity with pyrimethamine–sulfadiazine in early pregnancy

    • Significantly lowers vertical transmission rate

    • Licensed in Canada and Europe but available in the United States only via the Food and Drug Administration (FDA) using an IND

  • Prednisone
    • Indicated if active chorioretinitis threatens posterior pole or optic nerve, or if there is visually significant vitritis

    • Start 24–72 hours after antiparasitic therapy

Referral/Co-management

  • Infectious Disease

  • Ob-Gyn

  • Neurology

  • Cardiology

  • Pulmonology