An Introduction to Forensic Genetics by Goodwin, William, Linacre, Adrian, Hadi, Sibte -- Read -- Imperial Library of Trantor

Index

Cover Title Copyright Foreword Preface Preface to first edition 1: Introduction to forensic genetics

Forensic genetics A brief history of forensic genetics

2: DNA structure and the genome

DNA structure Organization of DNA into chromosomes The structure of the human genome Genetic diversity of modern humans The genome and forensic genetics Tandem repeats Single nucleotide polymorphisms WWW resources

3: Biological material -collection, characterization and storage

Sources of biological evidence Collection and handling of material at the crime scene Identification and characterization of biological evidence Evidence collection Sexual and physical assault Storage of biological material

4: DNA extraction and quantification

DNA extraction General principles of DNA extraction DNA extraction from challenging samples Quantification of DNA DNA IQ system

5: Polymerase chain reaction

The evolution of PCR-based profiling in forensic genetics DNA replication: the basis of the PCR The components of PCR Taq DNA polymerase The PCR process PCR inhibition Sensitivity and contamination The PCR laboratory

6: The analysis of short tandem repeats

Structure of STR loci The development of STR multiplexes Detection of STR polymorphisms Interpretation of STR profiles

7: Assessment of STR profiles

Stutter peaks Split peaks (±N) Pull-up Template DNA Overloaded profiles Low template DNA typing Peak balance Mixtures Degraded DNA PCR inhibition

8: Statistical interpretation of STR profiles

Population genetics Deviation from the Hardy–Weinberg equilibrium Statistical tests to determine deviation from the Hardy–Weinberg equilibrium Estimating the frequencies of STR profiles Corrections to allele frequency databases Which population frequency database should be used? Conclusions

9: Evaluation and presentation of DNA evidence

Hierarchies of propositions Likelihood ratios Two fallacies Comparison of three approaches

10: Databases of DNA profiles

The UK National DNA Database International situation

11: Kinship testing

Parentage testing Punnett square Identification of human remains

12: Single nucleotide polymorphisms

SNPs – occurrence and structure Detection of SNPs SNP detection for forensic applications Forensic applications of SNPs SNPs compared with STR loci

13: Lineage markers

Mitochondria Applications of mtDNA profiling Haplotypes and haplogroups The Y chromosome Forensic applications of Y chromosome polymorphisms

14: Non-human DNA typing

Non-human sample types Species identification Linkage to an individual using STR loci Linkage to an individual using mitochondrial loci Microbial DNA testing Concluding comments

Appendix A: Forensic parameters Appendix B: Useful web links Glossary Abbreviations Index Advertisment