Log In
Or create an account ->
Imperial Library
Home
About
News
Upload
Forum
Help
Login/SignUp
Index
Cover
Further Titles for Chromatographers
Title Page
Copyright
Preface
Introduction
Part I: Fundamentals
1.1 Chromatography
1.2 Chromatographic Figures of Merit
1.3 The Resolution of Two Peaks
1.4 Reduced Parameters
1.5 The Van Deemter Curve
1.6 Peak Capacity and Number of Possible Peaks
1.7 Statistical Resolution Probability: Simulation
1.8 Statistical Resolution Probability: Example
1.9 Precision and Accuracy of an Analytical Result
1.10 Standard Deviation
1.11 Variability of the Standard Deviation
1.12 Uncertainty Propagation
1.13 Reproducibility in Trace Analysis
1.14 Ruggedness
1.15 Calibration Curves
1.16 The HPLC Instrument
1.17 The Detector Response Curve
1.18 Noise
1.19 The Playground Presented as an Ishikawa Diagram
1.20 The Possible and the Impossible
Part II: Pitfalls and Sources of Error
2.1 Mixing of the Mobile Phase
2.2 Mobile Phase pH
2.3 Adjustment of Mobile Phase pH
2.4 Influence of the Acid Type and Concentration in the Eluent
2.5 Water as an Unintentional Additive in the Mobile Phase
2.6 Inadequate Purity of Mobile Phase Water
2.7 Inadequate Purity of a Mobile Phase Solvent
2.8 Inadequate Purity of a Mobile Phase Reagent
2.9 Incomplete Degassing
2.10 System Peaks and Quantitative Analysis
2.11 Sample Preparation with Solid Phase Extraction
2.12 Inadequate Stabilization of the Extraction Solvent
2.13 Poor Choice of Sample Solvent: Peak Distortion
2.14 Poor Choice of Sample Solvent: Tailing
2.15 Sample Solvent and Calibration Curve
2.16 Impurities in the Sample
2.17 Formation of a by-Product in the Sample Solution
2.18 Decomposition by the Sample Vial
2.19 Artifact Peaks from the Vial Septum
2.20 Formation of an Associate in the Sample Solution
2.21 Precision and Accuracy with Loop Injection
2.22 Injection Technique
2.23 Injection of Air
2.24 Sample Adsorption in the Loop
2.25 Extra-Column Volumes
2.26 Dwell Volume
2.27 Elution at t0
2.28 Classification of C18 Reversed Phases
2.29 Different Selectivity of C18 Reversed Phases
2.30 Different Batches of Stationary Phase
2.31 Chemical Reaction within the Column
2.32 Tailing of Phosphate Compounds in the Presence of Steel
2.33 Recovery and Peak Shape Problems with Proteins
2.34 Double Peaks from Stable Conformers
2.35 Influence of Temperature on the Separation
2.36 Thermal Non-Equilibrium within the Column
2.37 Influence of the Flow Rate on the Separation
2.38 Influence of Run Time and Flow Rate on Gradient Separations
2.39 UV Spectra and Quantitative Analysis
2.40 UV Detection Wavelength
2.41 Different Detection Properties of Diastereomers
2.42 Fluorescence Quenching by Air
2.43 Detector Overload in UV
2.44 Detector Overload in ELSD
2.45 Influence of the Retention Factor on Peak Height
2.46 Influence of the Flow Rate on Peak Area
2.47 Leaks in the HPLC Instrument
2.48 Impairment of Precision as a Result of Noise
2.49 Determination of Peak Area and Height at High Noise
2.50 Peak Height Ratios
2.51 Incompletely Resolved Peaks
2.52 Area Rules for Incompletely Resolved Peaks
2.53 Areas of a 1 : 10 Peak Pair
2.54 Heights of a 1 : 10 Peak Pair
2.55 Quantitative Analysis of a Small Peak
2.56 Incompletely Resolved Peaks with Tailing
2.57 Integration Threshold and Number of Detected Peaks
2.58 Detector Time Constant and Peak Shape
2.59 Quantitative Analysis in the 99% Range
2.60 Correlation Coefficient of Calibration Curves
Part III: Useful Strategies
3.1 Column Tests
3.2 Apparatus Tests
3.3 Wavelength Accuracy of the UV Detector
3.4 Internal Standards
3.5 A Linearity Test
3.6 Rules for Accurate Quantitative Peak Size Determination
3.7 High-Low Chromatography
3.8 Control Charts
3.9 Verification of the Analytical Result by Use of a Second Method
3.10 Description of Ruggedness
3.11 Rules for Passing on an HPLC Method
3.12 Quality Assurance in the Laboratory
3.13 Standard Operating Procedures
3.14 Method Validation
3.15 Some Elements of Validation
3.16 A Validation Example
3.17 System Suitability Test
3.18 From Repeatability to Reproducibility
3.19 Measurement Uncertainty
3.20 Formal Quality Assurance Systems
Index
← Prev
Back
Next →
← Prev
Back
Next →