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Index
THE ELEMENTS
OF
BACTERIOLOGICAL TECHNIQUE
A LABORATORY GUIDE FOR MEDICAL, DENTAL, AND TECHNICAL STUDENTS
BY
J. W. H. EYRE, M.D., M.S., F.R.S. (Edin.)
Director of the Bacteriological Department of Guy's Hospital, London, and Lecturer on Bacteriology in the Medical and Dental Schools; formerly Lecturer on Bacteriology at Charing Cross Hospital Medical School, and Bacteriologist to Charing Cross Hospital; sometime Hunterian Professor, Royal College of Surgeons, England
PREFACE TO THE SECOND EDITION
PREFACE TO THE FIRST EDITION
CONTENTS
BACTERIOLOGICAL TECHNIQUE.
I. LABORATORY REGULATIONS.
Guy's Hospital.
II. GLASS APPARATUS IN COMMON USE.
CLEANING OF GLASS APPARATUS.
PLUGGING TEST-TUBES AND FLASKS.
III. METHODS OF STERILISATION.
STERILISING AGENTS.
METHODS OF APPLICATION.
ELECTRIC SIGNAL TIMING CLOCK.
IV. THE MICROSCOPE.
METHODS OF MICROMETRY.
FOOTNOTES:
V. MICROSCOPICAL EXAMINATION OF BACTERIA AND OTHER MICRO-FUNGI.
APPARATUS AND REAGENTS USED IN ORDINARY MICROSCOPICAL EXAMINATION.
METHODS OF EXAMINATION.
Technique of Opening and Closing a Culture Tube.
VI. STAINING METHODS.
BACTERIA STAINS.
CONTRAST STAINS.
TISSUE STAINS.
BLOOD STAINS
METHODS OF DEMONSTRATING STRUCTURE OF BACTERIA, ETC.
DIFFERENTIAL METHODS OF STAINING.
VII. METHODS OF DEMONSTRATING BACTERIA IN TISSUES.
FREEZING METHOD.
PARAFFIN METHOD.
SPECIAL STAINING METHODS FOR SECTIONS.
VIII. CLASSIFICATION OF FUNGI.
MORPHOLOGY OF THE HYPHOMYCETES.
MORPHOLOGY OF THE BLASTOMYCETES.
IX. SCHIZOMYCETES.
ANATOMY.
PHYSIOLOGY.
X. NUTRIENT MEDIA.
MEAT EXTRACT.
STANDARDISATION OF MEDIA.
THE FILTRATION OF MEDIA.
STORING MEDIA IN BULK.
TUBING NUTRIENT MEDIA.
FOOTNOTES:
XI. CULTURE MEDIA.
ORDINARY OR STOCK MEDIA.
FOOTNOTES:
XII. SPECIAL MEDIA.
XIII. INCUBATORS.
FOOTNOTES:
XIV. METHODS OF CULTIVATION.
AEROBIC.
The Preparation of Plate Cultures.
ANAEROBIC CULTIVATIONS.
FOOTNOTES:
XV. METHODS OF ISOLATION.
A. By Mechanical Separation.
XVI. METHODS OF IDENTIFICATION AND STUDY.
SCHEME OF STUDY.
MACROSCOPICAL EXAMINATION OF CULTIVATIONS.
PATHOGENESIS.
IMMUNISATION.
ACTIVE IMMUNISATION.
THE PREPARATION OF HÆMOLYTIC SERUM.
THE TITRATION OF HÆMOLYTIC SERUM.
FOOTNOTES:
XVII. EXPERIMENTAL INOCULATION OF ANIMALS.
METHODS OF INOCULATION.
FOOTNOTES:
XVIII. THE STUDY OF EXPERIMENTAL INFECTIONS DURING LIFE.
COMPARATIVE HÆMOCYTOLOGY OF LABORATORY ANIMALS.
Sterilise by boiling.
AGGLUTININ.
OPSONIN.
IMMUNE BODY.
XIX. POST-MORTEM EXAMINATIONS OF EXPERIMENTAL ANIMALS.
XX. THE STUDY OF THE PATHOGENIC BACTERIA.
FOOTNOTES:
XXI. BACTERIOLOGICAL ANALYSES.
BACTERIOLOGICAL EXAMINATION OF WATER.
ANALYTICAL SCHEME FOR ISOLATION OF MEMBERS OF THE COLI AND TYPHOID GROUPS.
DIFFERENTIAL TABLE OF COLI-TYPHOID GROUP
EXAMINATION OF MILK.
ICE CREAM.
EXAMINATION OF CREAM AND BUTTER.
EXAMINATION OF UNSOUND MEATS.
A. Cultural Examination.
THE EXAMINATION OF OYSTERS AND OTHER SHELLFISH.
EXAMINATION OF SEWAGE AND SEWAGE EFFLUENTS.
EXAMINATION OF AIR.
EXAMINATION OF SOIL.
TESTING FILTERS.
TESTING OF DISINFECTANTS.
TABLE 27
APPENDIX.
METRIC AND IMPERIAL SYSTEMS OF WEIGHTS AND MEASURES.
TABLE FOR THE CONVERSION OF DEGREES CENTIGRADE INTO DEGREES FAHRENHEIT.
TABLE FOR THE CONVERSION OF DEGREES FAHRENHEIT INTO DEGREES CENTIGRADE.
Percentage Formula for addition of salts, etc., to completed media.
TABLES FOR PREPARING DILUTIONS
TABLES FOR PREPARING DILUTIONS.
TEMPERATURE PRESSURE TABLE.
TABLE FOR DESICCATION AT LOW TEMPERATURES IN VACUO.
ANTIFORMIN METHOD
INDEX
SAUNDERS' BOOKS
Pathology, Physiology
Histology, Embryology
Bacteriology, Biology
LITERARY SUPERIORITY
Mallory's
Pathologic Histology
REPRINTED IN THREE MONTHS
Howell's Physiology
THE NEW (5th) EDITION
Mallory and Wright's Pathologic Technique
Fifth Edition
Eyre's Bacteriologic Technic
JUST READY—NEW (2d) EDITION, REWRITTEN
McFarland's Pathology
THE NEW (2d) EDITION
McFarland's Biology: Medical and General
JUST READY—NEW (2d) EDITION
McFarland's Pathogenic Bacteria and Protozoa
NEW (7th) EDITION, ENLARGED
Hill's Histology and Organography
THE NEW (2d) EDITION
New (7th) Edition—5000 Sold in Two Months
IT DEFINES ALL THE NEW WORDS—IT IS UP TO DATE
Stengel's Text-Book of Pathology
Fifth Edition
Stiles' Nutritional Physiology
ILLUSTRATED
Jordan's General Bacteriology
NEW (3d) EDITION
Buchanan's Veterinary Bacteriology
THE BEST PUBLISHED
Heisler's Embryology
THIRD EDITION—WITH 212 ILLUSTRATIONS, 32 IN COLORS
Böhm, Davidoff, and Huber's Histology
SECOND EDITION, ENLARGED
Wells' Chemical Pathology
JUST READY—NEW (2d) EDITION
Lusk's Elements of Nutrition
THE NEW (2d) EDITION—TRANSLATED INTO GERMAN
Daugherty's Economic Zoölogy
ILLUSTRATED
Drew's Invertebrate Zoölogy
JUST READY—NEW (2d) EDITION
Norris' Cardiac Pathology
SUPERB ILLUSTRATIONS
McConnell's Pathology
NEW (2d) EDITION
Hektoen and Riesman's Pathology
Dürck and Hektoen's Special Pathologic Histology
Sobotta and Huber's Human Histology
INCLUDING MICROSCOPIC ANATOMY
Bosanquet on Spirochætes
ILLUSTRATED
Levy and Klemperer's Clinical Bacteriology
Lehmann, Neumann, and Weaver's Bacteriology
Dürck and Hektoen's General Pathologic Histology
American Text-Book of Physiology Second Edition
Warren's Pathology and Therapeutics Second Edition
Gorham's Bacteriology
Raymond's Physiology New (3d) Edition
Ball's Bacteriology Seventh Edition, Revised
Budgett's Physiology New (3d) Edition
Leroy's Histology New (4th) Edition
Barton and Wells' Medical Thesaurus
American Pocket Dictionary New (8th) Edition
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