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Index
Cover page Halftitle page Series page Title page Copyright page Contents List of illustrations Acknowledgements Chapter 1. No enzymes, no life
Beginnings and basics Can chemistry explain biology? The death of vitalism and the birth of biochemistry
Chapter 2. Making things happen—catalysis
Thermodynamics and kinetics Activation energy Reversibility and equilibrium Gaining insight from enzyme kinetics
Chapter 3. The chemical nature of enzymes
Isolation of enzymes Purification and controversy What are proteins? Amino acid sequence The shape of protein molecules Protein folding Forces in protein molecules
Chapter 4. Structure for catalysis
Structural complementarity—the lock-and-key model Enzyme-substrate complexes—fact or fancy? Catalytic groups The importance of flexibility Transition state analogues Assembling the cast Enzymes’ little helpers Catalytic power
Chapter 5. Enzymes in action
Proteinases in digestion Switching on: zymogens Stomachs of the cell Cell death Blood clotting Trapping useable chemical energy Translating the genetic code Isoenzymes
Chapter 6. Metabolic pathways and enzyme evolution
Proteins and evolution Comparison across biological species Enzyme families Divergence and convergence Where do new enzymes come from? How did metabolic pathways arise? Still further back
Chapter 7. Enzymes and disease
Enzymes in a medical context Enzymes for diagnosis Sick enzymes Enzymes, SIDS, and Jamaican Vomiting Sickness Enzymes as targets Aspirin Warfarin Penicillin Captopril Viral infection: HIV, coronaviruses, etc. Therapeutic enzymes Enzyme conjugates, ADEPT
Chapter 8. Enzymes as tools
Thinking beyond the original biological context Overcoming barriers to application Enzymes for washing Enzymes to make food Skin, hair, and feathers Enzymes for farming and waste treatment Enzymes for chemistry?
Chapter 9. Enzymes and genes—new horizons
Tailored enzymes—a possibility? Site-directed mutagenesis Random mutagenesis and screening Real targets: left- and right-handed molecules Amino acid dehydrogenases Enzymes, biological defence, and the genetic revolution
References Further reading Index Scientific Revolution Relativity Racism Planets The U.S Congress The European Union The American Presidency
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