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Index
Cover Page Title Page Copyright Page table of contents Acknowledgements Foreword Introduction Part One: The Importance of Yeast and Fermentation
A Brief History of Yeast Why Fermentation Is So Important Improving Fermentation Quality The Basics of Good Fermentation
Yeast Sugar Oxygen Nutrients Fermentation Systems Temperature Control Fermentation Monitoring
Part Two: Biology, Enzymes, and Esters
Yeast Biology
Genetics of S. cerevisiae Yeast Cell Structure Metabolism Alcohol Flocculation
Enzymes
How Do Enzymes Work? Enzymes in Malting Enzymes in Mashing Enzymes in Fermentation
Esters, Alcohols, and More
Esters Fusel Alcohols Diacetyl Organic Acids Sulfur Compounds Phenolic Compounds
Part Three: How to Choose the Right Yeast
Selection Criteria Beer Styles and Yeast Selection Yeast Strains
Ale Strain Overview Clean Ale Strains Fruity Ale Strains Hybrid Ale Strains Phenolic Ale Strains Eccentric Ale Strains Lager Strains
Multiple Strains in Your Brewery Multiple Strains in One Beer Brettanomyces
Contamination Concerns Brettanomyces Strains What Makes Brettanomyces Special? Inoculation Rates and Other Factors
Capturing Wild Yeast
Part Four: Fermentation
Fermentation Timeline
Lag Phase Exponential Growth Phase Stationary Phase
Wort Composition
Sugars Enzymes
Yeast Nutrition Aerating for Fermentation
The Need for Oxygen How Much Oxygen?
High-Gravity Beers Fermentation Systems
Homebrewing Fermentors Commercial Fermentors
Use of Antifoam Fermentation Temperatures
Fermentation Temperature Control Temperature Control for the Homebrewer
Optimizing Fermentation Flavor Fermentation Endgame
Attenuation Flocculation Diacetyl Rest Lagering
Bottle Conditioning Cask Conditioning
Part Five: Yeast Growth, Handling, and Storage
Pitching Rates Yeast Propagation Commercial Brewery Propagation Homebrew Propagation
Making a Starter What Is the Best Starter Size? Stepped Starters
Working With Dry Yeast Yeast Handling Yeast Collection
Top Cropping Top Cropping Timing and Techniques Bottom Cropping Bottom Cropping Timing and Techniques
Yeast Storage and Maintenance
Storage Vessels Shelf Life
Reusing Yeast
Viability and Vitality Revitalizing Rinsing Washing
Transporting Yeast
Part Six: Your Own Yeast Lab Made Easy
Quality From the Beginning Setting Up Your Lab
Environmental Considerations Lab Safety Lab Equipment How Much Lab Does My Brewery Need?
Sterilization
Wet Heat Dry Heat Incineration Tyndallization Autoclave Testing
Yeast Culturing
Plates and Slants Preparing Agar Slants and Plates Streaking a Plate Streaking a Slant Stabs Oil Immersion Water Immersion Freezing Picking Colonies Starting Propagation From a Plate Maintaining a Yeast Library
Yeast Capture
On the Road Bottled Beer
Yeast and Beer Quality Assurance
Plating Methods Membrane Filtration Pour Plates Spread Plates Check Plates Swabbing Tank Sampling Forced Wort Test Forced Ferment Test Diacetyl Force Broad Spectrum Method for VDK Fermentation Trials Yeast Strain Oxygen Demand Iodine Test for Glycogen Respiratory (Petite) Mutant Test YPD Medium
Bacteria Tests
UBA Medium HLP Medium SDA Medium MacConkey Medium Gram Stain
Wild Yeast Tests
LWYM or LCSM Lysine Media Wallerstein Media
Serial Dilution Cell Counting Viability
Methylene Blue (MB) Citrate Methylene Blue (CMB) Alkaline Methylene Blue (AMB) Alkaline Methylene Violet (AMV) Standard Plate Count (SPC)
Vitality
Acidification Power Test (AP)
Differentiation of Ale and Lager Yeast
By Growth at 37° C By Growth on Melibiose X-alpha-GAL Medium
Yeast Strain Differentiation
Giant Colony Multi-Strain Drift
Part Seven: Troubleshooting
Slow, Stuck, and Incomplete Fermentation
Fermentation Does Not Start No Activity After ‘X’ Hours Fermentation Does Not End Fermentation Seems Incomplete
Flocculation Changes Flavors and Aromas
Fruity Character and Fusel Alcohols Sulfur Phenols Acetaldehyde Diacetyl Sour Overly Sweet Overly Dry
Autolysis Carbonation
Lack of Carbonation Overcarbonation
Attenuation
Low Attenuation High Attenuation
Yeast Storage Problems
Declining or Low Yeast Viability Inadequate Shelf Life Washing Problems Rinsing Problems Transportation Problems
Propagation/Starter Problems Malt Contamination Troubleshooting Charts
List of Figures References Index
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