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Index
Cover Image
Table of Contents
Copyright
Foreword
Chapter 1. Quantitative PCR
Chapter 2. Signal Amplification Methods in Molecular Diagnostics
Chapter 3. The Lab-on-a-Chip Approach for Molecular Diagnostics
Chapter 4. Nucleic Extraction and Amplification
Chapter 5. General Procedures
I. Introduction
II. Gel Electrophoresis
III. Nucleic Acid Quantification by UV Absorbance
Chapter 6. Next-Generation Sequencing in Molecular Diagnostics
Chapter 7. In Situ Hybridization
I. Introduction
II. Components of ISH Hybridization Assays
III. Automation
IV. Troubleshooting
V. Applications
Chapter 8. Billing and Reimbursement for Molecular Diagnostics
Chapter 9. Molecular Pathology and Infectious Diseases
I. Introduction
II. Emerging Infectious Diseases
III. Biofilms
IV. Micrornas in Large DNA Viruses
V. Leishmania
VI. The Future
Chapter 10. Ethical Considerations in Molecular Diagnostics
Chapter 11. Quality Control, Assurance, Identification, and Standards
Chapter 12. Regulatory Issues in Molecular Diagnostics
Chapter 13. Thrombophilia
Chapter 14. Molecular Testing for Cystic Fibrosis and CFTR-Related Conditions
Chapter 15. Molecular Genetic Testing for X-linked Mental Retardation Disorders
Chapter 16. Hemochromatosis
Chapter 17. Standards and Recommendations for Molecular Diagnostic Testing for Huntington Disease, the Autosomal Dominant Spinocerebellar Ataxias, and Friedreich Ataxia
Chapter 18. The Role of Micro-RNAs in Cancer
Chapter 19. Molecular Diagnostics in the Evaluation of Cancer
I. Introduction and Modern Concepts of Cancer
II. Cancer Genetics
III. Epigenetics
IV. Prominent Targets of Molecular Tumor Markers
V. Nucleic Acid Molecular Markers for Cancer
VI. Rationale for Selection of Laboratory Tests in the Diagnosis of Cancer (Nakamura and Grody, 2004)
VII. Predictive Markers in Breast and Other Cancers (Duffy, 2005)
VIII. Examples of Molecular Markers for the early Detection of Cancer
IX. Molecular Diagnostic Assays of Specific Tumor Tissues
X. Linking Drug Response to Genetic Mutations of the Tumor
XI. Summary
Chapter 20. Gene Expression Analysis for Tumor Profiling
Chapter 21. Molecular Diagnostics of Hematopoietic Disorders
I. Introduction
II. Sample Collection and Preparation
III. Analytic Techniques
IV. Recommendations/Limitations of Available Methods
V. Special Considerations in Quality Management
VI. Reporting and Interpretation
VII. Clinical Indication and Rational
VIII. Case Studies
IX. Future Directions
X. Summary
Chapter 22. Molecular Diagnostics for the Hepatitis C Virus
Chapter 23. Molecular Diagnosis of Cytomegalovirus
I. Introduction and Definitions
II. Sample Collection and Preparation
III. Analytical Techniques
IV. Recommendations/Limitations of Available Methods
V. Special Considerations in Quality Management
VI. Reporting and Interpretation
VII. Clinical Indication and Rationale
VIII. Case Study
IX. Future Directions
X. Summary
Chapter 24. Molecular Detection of Multiple Respiratory Viruses
Chapter 25. Human Papillomavirus and its Role in Cervical Carcinoma
I. Introduction and Definitions
II. Sample Collection and Preparation
III. Analytical Techniques
IV. Recommendations/Limitations of Available Methods
V. Special Considerations in Quality Management
VI. Reporting and Interpretation
VII. Clinical Indication and Rationale
VIII. Future Directions
IX. Summary
Chapter 26. Molecular Methods for Identification and Characterization of Acinetobacter spp.
Chapter 27. Molecular Detection and Surveillance of Healthcare-Associated Infections
Chapter 28. Molecular Detection of Group B Streptococcus
Chapter 29. Role of Molecular Techniques in the Epidemiologic Investigation of Hospital-Acquired Infections
Chapter 30. Molecular HLA Typing Methods Used in Clinical Laboratories
Chapter 31. Identification Through DNA Analysis in Criminal and Family-Relatedness Investigations
Chapter 32. Overview of Pharmacogenomics and Applications for the Modern Clinical Laboratory
Chapter 33. UDP-Glucuronosyltransferase 1A1 and the Glucuronidation in Oncology Applications and Hyperbilirubinemia
I. Introduction
II. Analytical Techniques
III. Recommendation/Limitations of Available Methods
IV. Special Considerations in Quality Management
V. Reporting and Interpretation
VI. Clinical Indications and Rationale
VII. Future Directions
VIII. Summary
Chapter 34. Pharmacogenetics of Cytochrome P450 Enzymes
Chapter 35. Pharmacogenomics of Thiopurine Methyl Transferase (TPMT)
Chapter 36. Molecular Diagnostic Challenges of the Thalassemias
I. Introduction and Definitions
II. Sample Collection and Preparation
III. Analytical Techniques
IV. Recommendations/Limitations of Available Methods
V. Special Considerations in Quality Management
VI. Reporting, Interpretation, and Clinical Indication
VII. Case Studies
VIII. Future Directions
IX. Summary
Chapter 37. Herpes Simplex Virus
I. Sample Collection and Preparation
II. Analytical Techniques
III. Special Considerations in Quality Management
IV. Reporting Interpretation
V. Summary
Chapter 38. Molecular Diagnostic Evaluation of Deaf and Hard-of-Hearing Individuals
Index
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