Contents

Cover

Dedication

Title Page

Copyright

Acknowledgements

Chapter 1: Education and Training for the Personal Licence Holder

Introduction

Framework for Education and Training of Licence Holders

Aims of Accredited Training

Continuing Professional Development and Reflective Practice

Summary

References

Chapter 2: Ethical Considerations around using Animals in Research

History of the Use of Animals in Research

The Ethics of Using Animals in Experiments

The Local Ethical Review Process

The Principles of the Three RS

Public Perceptions

References

Chapter 3: The Regulatory Framework

Introduction to Legislation Regulating the Use of Animals in Research

The Animals (Scientific Procedures) Act 1986

Administration of the Act

Communication

Statistics of Animal Usage

Other Relevant Legislation

References

Chapter 4: Balancing Animal Welfare with Science

Welfare versus Science?

What is Welfare?

References

Chapter 5: Animal Husbandry, Environmental Enrichment and Occupational Health and Safety

Animal Husbandry

Environmental Enrichment

Supply and Transport

Aspects of Health, Safety and Security Affecting Animal Care

Acknowledgements

References and Further Reading

Chapter 6: Principles of Animal Use and Experimental Design

Principles of Experimental Design

Genetic and Welfare Monitoring of GA and HM Animals

References

Chapter 7: Handling and Techniques

Prior Considerations

Administration of Substances

Removal of Blood

Placement and Maintenance of Indwelling Cannulae

Arterial Sampling and Arterial Loops

Miscellaneous Procedures

Equipment for Blood Collection and Administration of Substances

References

Chapter 8: Humane Methods of Killing

Ethical Considerations in Killing Animals

Methods of Euthanasia

References

Chapter 9: Anaesthesia and Analgesia

Introduction

Pre-Anaesthetic Care and Evaluation

General Anaesthesia

Anaesthetic Management

Anaesthetic Emergencies

Muscle Relaxation During Anaesthesia

References

Chapter 10: Introduction to Surgery and Surgical Techniques

Pre-Surgical Preparation

Principles of Aseptic Technique

Summary of Lasa Guidelines on Rodent Surgery

Conduct of Surgical Procedures

Surgical Instruments

Suturing Techniques and Materials

Summary

References

Chapter 11: Small Laboratory Animals

Rodents

Mouse

Rat

Hamster

Gerbil

Guinea Pig

Rabbit

References

Chapter 12: Carnivores

Dog

Ferret

References

Chapter 13: Primates

Use of Primates in Research

New World Monkeys

Old World Monkeys

Health Monitoring, Quarantine and Common Diseases

Laboratory Procedures

Welfare

References

Chapter 14: Production (Farm) Animals

Poultry

Domestic Chicken

Ruminants

Sheep

Goats

Pigs

References and Further Reading

Chapter 15: Wild Animals

General Considerations

Legislation and Guidance

Project Planning

Catching and Trapping

Handling Wild Animals

Anaesthesia of Wild Animals

Identification of Wild Animals

Release of Animals Back to the Wild

References

Chapter 16: Aquatic Species

Introduction

Fish

Zebrafish

Rainbow Trout

Amphibians

References

Glossary

Index